Cryopreservation of Spin-Dried Mammalian Cells
Author Information
Author(s): Chakraborty Nilay, Menze Michael A., Malsam Jason, Aksan Alptekin, Hand Steven C., Toner Mehmet
Primary Institution: Massachusetts General Hospital, Harvard Medical School
Hypothesis
Can spin-drying improve the cryopreservation of mammalian cells by achieving vitrification without high concentrations of cryoprotectants?
Conclusion
The study demonstrated that spin-drying can effectively preserve mammalian cells at cryogenic temperatures with a significant survival rate.
Supporting Evidence
- 62% of the cells survived spin-drying with trehalose.
- 51% of the spin-dried cells survived after rehydration and showed normal growth.
- The spin-drying technique allows for rapid and uniform desiccation of cells.
Takeaway
Scientists found a new way to dry and store cells that helps them stay alive even when frozen, which is important for saving cells for later use.
Methodology
CHO cells were spin-dried to a low moisture content and then stored at cryogenic temperatures to assess survival and growth.
Limitations
The study primarily focused on CHO cells, and results may not be generalizable to other cell types.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website