Misregulation of SCM3 Causes Chromosome Instability
Author Information
Author(s): Mishra Prashant K., Au Wei-Chun, Choy John S., Kuich P. Henning, Baker Richard E., Foltz Daniel R., Basrai Munira A.
Primary Institution: National Cancer Institute, National Institutes of Health
Hypothesis
Overexpression of SCM3 and HJURP leads to defects in chromosome segregation in yeast and human cells.
Conclusion
The study concludes that stringent regulation of HJURP and SCM3 expression is critical for genome stability.
Supporting Evidence
- Overexpression of SCM3 or HJURP caused chromosome loss in yeast.
- Overexpression of HJURP in human cells led to mitotic defects.
- The N-terminus of Scm3p is essential for its centromeric association.
Takeaway
When certain proteins that help with chromosome separation are too high, it can cause problems, like losing chromosomes, which is bad for cells.
Methodology
The study involved overexpressing SCM3 and HJURP in yeast and human cells, followed by assays to measure chromosome loss and defects in cell division.
Limitations
The study primarily focuses on yeast models, which may not fully replicate human cellular behavior.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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