Automated Sample Preparation for HPLC
Author Information
Author(s): J. D. H. Cooper, D. C. Turnell
Primary Institution: Department of Biochemistry, Coventry and Warwickshire Hospital
Hypothesis
The incorporation of dialysis and trace enrichment in an automated sample preparation system will improve the analysis of biological fluids prior to HPLC.
Conclusion
The automated sample preparation system effectively analyzes therapeutic concentrations of phenobarbitone in human serum.
Supporting Evidence
- The system allows for the direct sampling of complex biological samples.
- Chromatograms from the automated system showed no interfering peaks.
- The procedure was linear up to 100 mg/1 of phenobarbitone in serum.
- The coefficient of variation for the assay was low at 1.5% and 1.4%.
Takeaway
This study shows a new way to prepare samples for testing that makes it easier to find drugs in blood without messing things up.
Methodology
An automated system using dialysis and trace enrichment was developed and tested for analyzing phenobarbitone in human serum using HPLC.
Limitations
The efficiency of dialysis is not optimal, and analyte recoveries may be less than 50% without further modifications.
Participant Demographics
Human serum samples were used for analysis.
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