Gene Expression Microarray for Gilthead Sea Bream
Author Information
Author(s): Serena Ferraresso, Nicola Vitulo, Alba N Mininni, Chiara Romualdi, Barbara Cardazzo, Enrico Negrisolo, Richard Reinhardt, Adelino VM Canario, Tomaso Patarnello, Luca Bargelloni
Primary Institution: University of Padova
Hypothesis
Can a reliable oligo-microarray platform be developed for the gilthead sea bream to analyze gene expression?
Conclusion
A highly reliable oligo-microarray platform was developed and validated for the gilthead sea bream, allowing for future expansions as new transcripts are identified.
Supporting Evidence
- The microarray platform showed high reproducibility with correlation coefficients always > 0.99.
- 1,050 differentially expressed genes were identified between the two developmental stages.
- Functional analysis indicated that down-regulated genes were mostly essential/housekeeping genes.
Takeaway
Scientists created a special tool to study how genes work in a type of fish called gilthead sea bream, which can help improve fish farming.
Methodology
The study involved constructing a database of unique transcripts and designing two probes for each transcript, followed by validation through microarray analysis and qRT-PCR.
Limitations
The study's findings are based on limited knowledge of the species transcriptome and may not cover all aspects of gene expression.
Participant Demographics
Larvae of gilthead sea bream at two developmental stages (1-day and 4-days old).
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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