Genotoxic Effects of Titanium Dioxide Nanoparticles and Fullerene in Mouse Cells
Author Information
Author(s): Xu An, Chai Yunfei, Nohmi Takehiko, Hei Tom K
Primary Institution: Columbia University
Hypothesis
What are the genotoxic responses of titanium dioxide nanoparticles and fullerene in gpt delta transgenic mouse primary embryo fibroblasts?
Conclusion
Both titanium dioxide nanoparticles and fullerene induce kilo-base pair deletion mutations in mouse cells, mediated by peroxynitrite anions.
Supporting Evidence
- Both TiO2 nanoparticles and C60 increased mutation yield in mouse cells.
- Endocytosis inhibitors reduced the mutation frequency induced by TiO2 and C60.
- ONOO- production was significantly increased in cells treated with TiO2 and C60.
Takeaway
Tiny particles called titanium dioxide and fullerene can change the DNA in mouse cells, which might be harmful.
Methodology
The study used gpt delta transgenic mouse primary embryo fibroblasts to assess mutation frequencies after exposure to titanium dioxide nanoparticles and fullerene.
Limitations
The study primarily focuses on in vitro results, which may not fully represent in vivo effects.
Participant Demographics
Transgenic mouse primary embryo fibroblasts were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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