NleC: A Bacterial Effector That Degrades NF-κB
Author Information
Author(s): Jaclyn S. Pearson, Patrice Riedmaier, Olivier Marchès, Gad Frankel, Elizabeth L. Hartland
Primary Institution: University of Melbourne
Hypothesis
Can NleC from enteropathogenic Escherichia coli inhibit NF-κB activation?
Conclusion
NleC is a type III effector protease that degrades NF-κB, contributing to the inhibition of innate immune activation.
Supporting Evidence
- NleC was shown to induce degradation of the NF-κB subunit p65.
- NleC inhibited IL-8 production during prolonged EPEC infection.
- A double nleE/nleC mutant was more impaired in inhibiting IL-8 secretion than single mutants.
Takeaway
NleC is like a tiny ninja that sneaks into cells and breaks down a part of the immune system called NF-κB, helping bacteria avoid being attacked.
Methodology
The study involved screening T3SS effectors for their ability to inhibit p65 nuclear translocation and assessing their effects on IL-8 secretion.
Limitations
The study primarily focused on in vitro experiments, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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