Universal Stress Proteins Are Important for Oxidative and Acid Stress Resistance and Growth of Listeria monocytogenes EGD-e In Vitro and In Vivo
2011

Universal Stress Proteins in Listeria monocytogenes

publication Evidence: high

Author Information

Author(s): Seifart Gomes Christa, Izar Benjamin, Pazan Farhad, Mohamed Walid, Mraheil Mobarak Abu, Mukherjee Krishnendu, Billion André, Aharonowitz Yair, Chakraborty Trinad, Hain Torsten

Primary Institution: Institute of Medical Microbiology, Justus-Liebig-University, Giessen, Germany

Hypothesis

Universal stress proteins are important for the oxidative and acid stress resistance and growth of Listeria monocytogenes.

Conclusion

Universal stress proteins are crucial for the survival and growth of Listeria monocytogenes under stress conditions.

Supporting Evidence

  • Deletion mutants showed reduced growth under acidic and oxidative stress.
  • Survival rates of mutants were significantly lower in murine macrophages compared to wild-type.
  • Virulence was impaired in invertebrate models when usp genes were deleted.

Takeaway

This study shows that certain proteins help Listeria survive tough conditions, like being in acidic environments or facing harmful substances.

Methodology

The study involved creating deletion mutants of universal stress proteins and testing their growth and survival under various stress conditions in vitro and in vivo.

Limitations

The study did not explore the effects of universal stress proteins on biofilm formation or resistance to antibiotics.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024965

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication