Use of a mitochondrial COI sequence to identify species of the subtribe Aphidina (Hemiptera, Aphididae)
2011
Identifying Aphid Species Using DNA
Sample size: 198
publication
Evidence: moderate
Author Information
Author(s): Jian-Feng Wang, Li-Yun Jiang, Ge-Xia Qiao
Primary Institution: Institute of Zoology, Chinese Academy of Sciences
Hypothesis
Can mitochondrial COI sequences effectively identify species within the subtribe Aphidina?
Conclusion
The study found that mitochondrial COI sequences are highly effective for identifying species within the Aphidina subtribe.
Supporting Evidence
- Thirty-six species of Aphidina were identified using DNA sequences.
- Mean intraspecific sequence divergence was found to be 0.52%.
- Mean interspecific divergence was 6.80%, indicating significant variation among species.
Takeaway
Scientists used a part of DNA to tell different types of tiny bugs called aphids apart, which helps farmers know which ones are pests.
Methodology
The study analyzed 198 COI sequences from aphid specimens, using PCR and sequencing to identify species relationships.
Limitations
The study's findings are based on a limited number of specimens and may not cover all species diversity.
Digital Object Identifier (DOI)
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