How Serotonin Affects the Transporter in Cells
Author Information
Author(s): Ahmed Billow A., Bukhari Irfan A., Jeffus Brandon C., Harney Justin T., Thyparambil Sheeno, Ziu Endrit, Fraer Mony, Rusch Nancy J., Zimniak Piotr, Lupashin Vladimir, Tang Dale, Kilic Fusun
Primary Institution: The University of Arkansas for Medical Sciences
Hypothesis
Does serotonin stimulation affect the association between vimentin and the serotonin transporter (SERT)?
Conclusion
Serotonin stimulation enhances the association between vimentin and SERT, altering SERT's cellular distribution.
Supporting Evidence
- 5HT-stimulation accelerates the translocation of SERT from the plasma membrane.
- Truncation of the C-terminus of SERT affects its association with vimentin.
- Phosphorylation of vimentin alters its interaction with SERT.
Takeaway
When serotonin levels go up, it helps a protein called vimentin stick to another protein called SERT, which changes where SERT is in the cell.
Methodology
The study used biochemical and proteomic analyses to investigate the interaction between SERT and vimentin in response to serotonin stimulation.
Limitations
The study primarily focuses on in vitro models, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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