Single Immunization with HA DNA Vaccine Provides Early Protection Against H5N1 in Mice
Author Information
Author(s): Zheng Liyun, Wang Fuyan, Yang Zhongdong, Chen Jianjun, Chang Haiyan, Chen Ze
Primary Institution: Wuhan Institute of Virology, Chinese Academy of Sciences
Hypothesis
Can a single immunization with HA DNA vaccine provide early protection against H5N1 avian influenza virus in mice?
Conclusion
A single immunization of 100 μg H5 HA DNA vaccine combined with electroporation was able to provide early protection in mice against homologous virus infection.
Supporting Evidence
- Immunization with HA DNA induced specific IgM and IgG antibodies in mice.
- Mice immunized 5 days before viral challenge showed a survival rate of 50%, 83%, and 100% at dosages of 10 μg, 50 μg, and 100 μg, respectively.
- Significant amounts of IFN-γ-producing cells were detected in all immunized groups.
- The protective abilities were correlated with the amount of injected DNA and the time after vaccination.
Takeaway
The study shows that giving a single shot of a special vaccine can help mice fight off a dangerous flu virus quickly.
Methodology
Mice were immunized with HA DNA at different dosages and challenged with a lethal dose of H5N1 virus; survival rates and immune responses were measured.
Limitations
The study was conducted in mice, and the results may not directly translate to humans.
Participant Demographics
Female BALB/c mice aged 6–8 weeks.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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