How MicroRNA-744 Regulates TGF-β1
Author Information
Author(s): Martin John Jenkins, Robert H. Bennagi, Rasha Krupa, Aleksandra Phillips, Aled O. Bowen, Timothy Fraser, Donald J.
Primary Institution: School of Medicine, Institute of Nephrology, Cardiff University, Cardiff, Wales, United Kingdom
Hypothesis
The study investigates the role of the TGF-β1 3′Untranslated Region (3′UTR) and its regulation by microRNA-744.
Conclusion
MicroRNA-744 directly targets the short isoform of the TGF-β1 3′UTR, leading to decreased TGF-β1 synthesis.
Supporting Evidence
- Both long and short TGF-β1 3′UTR variants were expressed in HK-2 cells.
- Transfection with miR-744 precursor led to a significant decrease in TGF-β1 release.
- miR-744 was confirmed to repress TGF-β1 synthesis experimentally.
Takeaway
This study shows that a tiny molecule called microRNA-744 can stop a protein called TGF-β1 from being made, which is important for many body processes.
Methodology
The study used luciferase reporter assays and qRT-PCR to analyze the effects of microRNA-744 on TGF-β1 expression.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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