Fast Method for Assessing Heart Damage in Small Animals
Author Information
Author(s): Anthony N Price, King K Cheung, Shiang Y Lim, Derek M Yellon, Derek J Hausenloy, Mark F Lythgoe
Primary Institution: UCL Centre for Advanced Biomedical Imaging, University College London, UK
Hypothesis
Can a new imaging protocol improve the assessment of myocardial infarction in small animals?
Conclusion
The new imaging protocol provides a reliable and efficient method for assessing myocardial infarction in small animals.
Supporting Evidence
- The imaging protocol was validated against triphenyltetrazolium chloride staining in rats.
- Clear areas of enhancement were observed in LGE images from all animals.
- Typical inversion times required to null healthy myocardium were established for both rats and mice.
Takeaway
This study shows a quick way to take pictures of heart damage in small animals, helping scientists understand heart problems better.
Methodology
The study used a multi-slice inversion recovery gradient echo sequence to assess late gadolinium enhancement in rats and mice after inducing myocardial infarction.
Limitations
The study did not perform TTC staining in mice due to their involvement in another long-term study.
Participant Demographics
Wistar rats (9 males, aged 7-8 weeks) and B6Sv129 mice (6 males, 9-11 weeks old).
Digital Object Identifier (DOI)
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