Characterization of a Phage Targeting E. coli O157:H7
Author Information
Author(s): Kutter Elizabeth M, Skutt-Kakaria Kyobi, Blasdel Bob, El-Shibiny Ayman, Castano Anna, Bryan Daniel, Kropinski Andrew M, Villegas Andre, Ackermann Hans-Wolfgang, Toribio Ana L, Pickard Derek, Anany Hany, Callaway Todd, Brabban Andrew D
Primary Institution: The Evergreen State College, Olympia, WA, USA
Hypothesis
Phage vB_EcoM_CBA120 is a specific bacteriophage that can effectively target and infect Escherichia coli O157:H7.
Conclusion
Phage CBA120 is highly specific for E. coli O157:H7 and exhibits unique genomic and physiological characteristics compared to other phages.
Supporting Evidence
- CBA120 is the first phage identified that specifically targets E. coli O157:H7.
- The genome of CBA120 is highly similar to that of the Salmonella phage ViI.
- CBA120 exhibits unique tail structures compared to other T4-like phages.
Takeaway
Scientists found a special virus that can attack a harmful bacteria called E. coli O157:H7, which is important for food safety.
Methodology
The study involved isolating and characterizing the phage CBA120 from cattle feedlots, followed by genomic sequencing and physiological experiments.
Limitations
The study primarily focused on a single phage and its interactions with a limited number of bacterial strains.
Digital Object Identifier (DOI)
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