mRNA expression of the DNA replication-initiation proteins in epithelial dysplasia and squamous cell carcinoma of the tongue
2008

DNA Replication Proteins as Biomarkers for Tongue Cancer

Sample size: 64 publication Evidence: high

Author Information

Author(s): Li Jian-na, Feng Chong-jin, Lu Yong-jun, Li Hui-jun, Tu Zheng, Liao Gui-qing, Liang Chun

Primary Institution: Sun Yat-sen University

Hypothesis

DNA replication-initiation proteins are useful biomarkers for early detection and diagnosis of squamous cell carcinoma (SCC) of the tongue.

Conclusion

The study found that mRNA levels of certain DNA replication-initiation proteins are higher in malignant lesions compared to precancerous conditions, indicating their potential as biomarkers for early detection of SCC.

Supporting Evidence

  • The expression levels of CDC6, CDT1, MCM2, and CDC45 were significantly higher in malignant SCC than in mild precancerous dysplasia.
  • A strong association was found between CDC6 and CDT1, and between MCM2 and CDC45 expression.
  • MCM2 and CDC45 showed higher accuracy for distinguishing dysplasia from SCC compared to CDC6 and CDT1.

Takeaway

Scientists looked at special proteins in tongue tissue to see if they can help find cancer early. They found that higher levels of these proteins mean a higher chance of cancer.

Methodology

The study analyzed mRNA expression of CDC6, CDT1, MCM2, and CDC45 in tongue tissues using quantitative real-time PCR.

Participant Demographics

Median age of participants was 54 years, with a range from 17 to 82 years; 52% were male and 48% were female.

Statistical Information

P-Value

p = 0.043

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2407-8-395

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