KSHV ORF45 and Kinesin-2 in Viral Transport
Author Information
Author(s): Sathish Narayanan, Zhu Fan, Xiu Yuan, Yan Yuan
Primary Institution: Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
Hypothesis
Does the KSHV ORF45 protein interact with KIF3A to facilitate the transport of viral capsid-tegument complexes along microtubules?
Conclusion
The study demonstrates that KSHV ORF45 interacts with KIF3A, which is crucial for the transport of viral complexes along microtubules, impacting viral egress.
Supporting Evidence
- ORF45 was found to interact with KIF3A, a kinesin-2 motor protein.
- Microtubules were essential for the transport of KSHV viral complexes.
- Inhibition of KIF3A-ORF45 interaction reduced KSHV egress.
- Nocodazole treatment decreased viral titers, indicating the importance of microtubules.
Takeaway
This study shows that a protein from the KSHV virus helps it move inside cells by grabbing onto a motor protein, which acts like a train on tracks.
Methodology
The study used yeast two-hybrid screening, co-immunoprecipitation, and confocal microscopy to analyze the interaction between ORF45 and KIF3A.
Limitations
The study does not fully explore the role of other kinesins in KSHV transport and the potential off-target effects of shRNA knockdown.
Digital Object Identifier (DOI)
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