Lentiviral Gene Transfer into Dorsal Root Ganglion of Adult Rats
Author Information
Author(s): Yu Hongwei, Fischer Greg, Jia Guangfu, Reiser Jakob, Park Frank, Hogan Quinn H
Primary Institution: Medical College of Wisconsin
Hypothesis
Can modified lentiviral vectors efficiently transduce sensory neurons in the dorsal root ganglion (DRG) of adult rats?
Conclusion
The study demonstrates that lentiviral vectors can effectively transduce sensory neurons in the DRG, showing potential for gene delivery in pain research and treatment.
Supporting Evidence
- Lentivectors showed high transduction efficiency in vitro with 95% success in specific conditions.
- Transduction rates in vivo were 19 ± 9% and 20 ± 8% for different lentivector generations.
- Transduced neurons included various types, indicating broad applicability for gene delivery.
Takeaway
Researchers used a special virus to deliver genes into nerve cells in rats to help understand and treat pain better.
Methodology
The study involved creating modified lentiviral vectors and testing their ability to transduce neurons in vitro and in vivo in adult rats.
Limitations
The study primarily focused on a specific type of gene delivery and did not explore long-term effects or other potential delivery methods.
Participant Demographics
Adult Sprague Dawley rats, 5-6 weeks old.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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