Impact of CD1d Deficiency on Metabolism
Author Information
Author(s): Maya E. Kotas, Hui-Young Lee, Matthew P. Gillum, Charles Annicelli, Blas A. Guigni, Gerald I. Shulman, Ruslan Medzhitov
Primary Institution: Yale University School of Medicine
Hypothesis
Lipid presentation by CD1d could report on metabolic status and engage iNKTs to regulate cellular lipid content through their various effector mechanisms.
Conclusion
CD1d deficiency leads to mild exacerbation of hepatic steatosis and glucose intolerance during high fat feeding, suggesting a role for CD1d in metabolic regulation.
Supporting Evidence
- CD1d deficiency led to increased hepatic triglyceride accumulation.
- CD1dā/ā mice showed worsened glucose intolerance compared to controls.
- iNKTs were selectively decreased in the livers of obese mice.
Takeaway
Mice without CD1d, a molecule that helps immune cells sense fats, had more fat in their livers and had trouble managing sugar levels when they ate a high-fat diet.
Methodology
The study involved examining CD1d deficient mice under various metabolic stress conditions, including high fat feeding and choline-deficient diets.
Potential Biases
Potential genetic differences between mouse strains could influence results.
Limitations
The study's findings may not be generalizable beyond the specific metabolic challenges tested.
Participant Demographics
Mice used in the study included CD1dā/ā and wild-type controls, with various strains maintained under controlled conditions.
Statistical Information
P-Value
0.19
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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