Erythrocyte transketolase activity measured on the Gemstar discrete analyser
1984
Measuring Erythrocyte Transketolase Activity with Gemstar Analyser
Sample size: 22
publication
Evidence: moderate
Author Information
Author(s): J. E. Buttery, B. R. Chamberlain, C. R. Milner
Primary Institution: The Queen Elizabeth Hospital
Conclusion
The Gemstar analyser provides comparable results to the manual method for measuring erythrocyte transketolase activity.
Supporting Evidence
- The correlation for the TK assay between Gemstar and manual methods was r=0.98.
- The mean TK activity measured by Gemstar was 0.84 U/g Hb compared to 0.82 U/g Hb by the manual method.
- The reference range for erythrocyte TK activity on the Gemstar is 0.6 to 1.3 U/g Hb.
Takeaway
This study shows a new machine can measure a specific enzyme in blood samples just as well as the old method, but faster.
Methodology
The study adapted a kinetic assay for erythrocyte transketolase to the Gemstar analyser and compared it with a manual method.
Limitations
The day-to-day precision could not be performed due to specimen instability.
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