A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides
2011

New Method for Producing Microbial Oil from Mucor circinelloides

publication Evidence: moderate

Author Information

Author(s): Xia Chunjie, Zhang Jianguo, Zhang Weidong, Hu Bo

Primary Institution: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China

Hypothesis

Can pH adjustment during cell growth enhance pellet formation and lipid accumulation in Mucor circinelloides?

Conclusion

The study found that pH adjustment is crucial for inducing cell pelletization in Mucor circinelloides, which does not significantly affect cell growth or lipid production.

Supporting Evidence

  • Pelletized fungal cells can be easily separated from the broth by simple filtration.
  • Cell growth and lipid production were not significantly affected by pelletization.
  • High carbon-to-nitrogen ratios and high temperatures triggered lipid accumulation.

Takeaway

Scientists discovered that changing the acidity of the growth environment helps a fungus make little balls of cells that are easier to collect and produce oil for biodiesel.

Methodology

The study involved growing Mucor circinelloides in flasks under various pH conditions to observe effects on cell growth and lipid accumulation.

Limitations

The study did not explore the economic feasibility of the new method for large-scale biodiesel production.

Digital Object Identifier (DOI)

10.1186/1754-6834-4-15

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