How Ghrelin Activates Akt Signaling
Author Information
Author(s): Lodeiro Maria, Theodoropoulou Marily, Pardo Maria, Casanueva Felipe F., Camiña Jesus P.
Primary Institution: Instituto de Investigaciones Sanitarias, Complejo Hospitalario Universitario de Santiago (CHUS), Santiago de Compostela, Spain
Hypothesis
The study aims to identify the signaling mechanisms involved in ghrelin-stimulated activation of the serine/threonine kinase Akt.
Conclusion
The study reveals that c-Src is crucially involved in the ghrelin-mediated activation of Akt through both G protein-dependent and β-arrestin-dependent mechanisms.
Supporting Evidence
- Ghrelin activates Akt through distinct signaling mechanisms involving G proteins and β-arrestins.
- c-Src is essential for the phosphorylation of Akt in response to ghrelin.
- PDK1 and mTORC2 are crucial for the phosphorylation of Akt.
Takeaway
Ghrelin helps activate a protein called Akt, which is important for cell growth and metabolism, using two different pathways.
Methodology
The study used HEK293 cells and 3T3-L1 preadipocyte cells to assess the roles of G proteins and β-arrestins in Akt activation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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