How Two Polycomb Complexes Control Muscle Cell Differentiation
Author Information
Author(s): Stojic Lovorka, Jasencakova Zuzana, Prezioso Carolina, Stützer Alexandra, Bodega Beatrice, Pasini Diego, Klingberg Rebecca, Mozzetta Chiara, Margueron Raphael, Puri Pier Lorenzo, Schwarzer Dirk, Helin Kristian, Fischle Wolfgang, Orlando Valerio
Primary Institution: Dulbecco Telethon Institute, IRCCS Fondazione Santa Lucia, Rome, Italy
Hypothesis
The dynamics of PRC2 complexes during skeletal muscle differentiation are crucial for myogenin activation.
Conclusion
The study reveals that the PRC2-Ezh1 complex is essential for the proper timing of myogenin transcriptional activation during muscle differentiation.
Supporting Evidence
- PRC2-Ezh2 is predominant in proliferating myoblasts, while PRC2-Ezh1 is specific for post-mitotic myotubes.
- Ezh1 depletion impairs muscle differentiation and MyoD recruitment.
- The dynamics of PRC2 complexes are crucial for the correct timing of myogenin transcriptional activation.
- Msk1-dependent phosphorylation of H3S28 is critical for the displacement of PRC2-Ezh2.
Takeaway
There are two important protein complexes that help muscle cells grow and change. One complex helps start the process, while the other helps finish it.
Methodology
The study used C2C12 mouse myoblasts to analyze the dynamics of PRC2 complexes during differentiation through various experiments including chromatin immunoprecipitation (ChIP) and RNA analysis.
Digital Object Identifier (DOI)
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