Interaction between Shrimp Protein PmFKBP46 and WSSV VP15
Author Information
Author(s): Sangsuriya Pakkakul, Senapin Saengchan, Huang Wei-Pang, Lo Chu-Fang, Flegel Timothy W.
Primary Institution: Mahidol University, Bangkok, Thailand
Hypothesis
The major nucleocapsid protein VP15 of WSSV interacts with a novel shrimp protein PmFKBP46.
Conclusion
The study suggests that PmFKBP46 may play a role in genome packaging by interacting with the viral protein VP15 during WSSV assembly.
Supporting Evidence
- VP15 is involved in packaging the WSSV genome during virion formation.
- PmFKBP46 was identified as a protein that interacts with VP15 using yeast two-hybrid analysis.
- Both proteins were shown to co-localize in the nuclei of shrimp hemocytes.
- Electrophoretic mobility shift assays confirmed that both proteins have DNA-binding activity.
Takeaway
Researchers found a shrimp protein that works with a virus protein to help the virus make copies of itself.
Methodology
The study used yeast two-hybrid screening, GST pull-down assays, and electrophoretic mobility shift assays to investigate protein interactions.
Limitations
The study does not confirm the role of PmFKBP46 in the WSSV replication cycle.
Digital Object Identifier (DOI)
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