The SET Complex and Its Role in HIV-1 Autointegration
Author Information
Author(s): Yan Nan, Cherepanov Peter, Daigle Janet E., Engelman Alan, Lieberman Judy
Primary Institution: Immune Disease Institute and Department of Pediatrics, Harvard Medical School
Hypothesis
How does the SET complex protect HIV-1 from autointegration?
Conclusion
The SET complex facilitates HIV-1 infection by preventing autointegration.
Supporting Evidence
- Knocking down SET complex proteins reduced HIV-1 infectivity by 3 to 4-fold.
- SET complex proteins were shown to bind to HIV-1 DNA in the cytoplasm.
- Autointegration increased 2.5-fold in cells with knocked down SET complex proteins.
Takeaway
The SET complex helps HIV-1 by stopping it from accidentally integrating into itself, which would end the infection.
Methodology
The study involved knocking down components of the SET complex in HeLaCD4 cells and measuring the effects on HIV-1 infection and integration.
Limitations
The study primarily used HeLaCD4 cells, which may not fully represent the behavior of HIV-1 in primary immune cells.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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