How Local Anesthetics Activate TRPA1
Author Information
Author(s): Andreas Leffler, Anja Lattrell, Sergej Kronewald, Florian Niedermirtl, Carla Nau
Primary Institution: Friedrich-Alexander-University Erlangen-Nuremberg
Hypothesis
The study aimed to characterize essential structural determinants for local anesthetic-evoked activation of TRPA1.
Conclusion
Local anesthetics activate TRPA1 through distinct mechanisms compared to other agonists like menthol.
Supporting Evidence
- Lidocaine activates TRPA1 in a concentration-dependent manner.
- The EC50-value for lidocaine-evoked activation of rodent TRPA1 is 5.7 ± 0.2 mM.
- Lidocaine-activated TRPA1-currents are blocked by the TRPA1-antagonist HC-030031.
Takeaway
Local anesthetics can make certain nerve cells more active, which is different from how they usually work to numb pain.
Methodology
The study used whole-cell patch clamp recordings on recombinant rodent and human TRPA1 expressed in HEK293t cells.
Limitations
The study primarily focused on in vitro experiments, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website