VEGFR2 Regulates Its Own Transcription
Author Information
Author(s): Domingues Inês, Rino José, Demmers Jeroen A. A., de Lanerolle Primal, Santos Susana Constantino Rosa
Primary Institution: Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal
Hypothesis
Does VEGFR2 act as a transcription factor to regulate its own expression?
Conclusion
Nuclear VEGFR2 can bind to and activate its own promoter, suggesting a mechanism for amplifying the angiogenic response.
Supporting Evidence
- VEGFR2 binds to the Sp1-responsive region of its own promoter.
- Nuclear VEGFR2 interacts with several nuclear proteins, including Sp1.
- VEGFR2's DNA binding is dependent on its activation by VEGF.
- Blocking VEGFR2 activation reduces its binding to its own promoter.
Takeaway
VEGFR2, a protein involved in blood vessel formation, can move into the cell nucleus and help make more of itself, which could help in healing and growth.
Methodology
The study used chromatin immunoprecipitation (ChIP) assays, EMSA assays, and luciferase reporter assays to investigate VEGFR2's role in transcription.
Limitations
The study does not identify the specific DNA sequence that VEGFR2 binds to in the promoter.
Statistical Information
P-Value
0.007
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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