Study of Aspergillus niger's Response to Different Sugars
Author Information
Author(s): Jørgensen Thomas R, Goosen Theo, van den Hondel Cees AMJJ, Ram Arthur FJ, Iversen Jens JL
Primary Institution: University of Southern Denmark
Hypothesis
Can carbon source dependent enhancement of protein secretion lead to upregulation of secretory pathway elements beyond those involved in N-glycosylation?
Conclusion
The study shows that upregulation of secretory pathway genes occurs in conditions inducing secretion of endogenous glycoproteins, indicating a general mechanism for modulating secretion capacity.
Supporting Evidence
- The production rate of extracellular proteins was about three times higher on maltose compared to xylose.
- More than 90 genes related to protein secretion were upregulated when A. niger was grown on maltose.
- The study demonstrated that the transcriptional regulation of protein synthesis and secretory pathway genes reflects a general mechanism for modulation of secretion capacity.
Takeaway
This study found that when a fungus called Aspergillus niger eats different sugars, it can produce more proteins and change how it makes them.
Methodology
The study used carbon-limited chemostat cultures to compare the transcriptomic profiles of A. niger growing on xylose and maltose.
Statistical Information
P-Value
0.005
Statistical Significance
p<0.005
Digital Object Identifier (DOI)
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