The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model
2009

Effects of DNA Vaccine on Lung Cancer Treatment

Sample size: 6 publication 10 minutes Evidence: moderate

Author Information

Author(s): Lai Ming-Derg, Yen Meng-Chi, Lin Chiu-Mei, Tu Cheng-Fen, Wang Chun-Chin, Lin Pei-Shan, Yang Huei-Jiun, Lin Chi-Chen

Primary Institution: National Cheng Kung University, Taiwan

Hypothesis

How do different routes of administration and formulations of a xenogenic EGFR DNA vaccine affect its therapeutic efficacy in a lung cancer model?

Conclusion

Gene gun administration of a non-coating xenogenic EGFR DNA vaccine may be the preferred method for treating EGFR-positive lung tumors.

Supporting Evidence

  • Gene gun administration of non-coating DNA vaccine generated the strongest T cell activity.
  • CD8(+) T cells were essential for anti-tumor immunity.
  • Non-coating DNA vaccine administered by gene gun delayed tumor growth significantly compared to control.

Takeaway

This study tested different ways to give a cancer vaccine to mice and found that one method worked best at fighting lung cancer.

Methodology

Mice were immunized with a xenogenic EGFR DNA vaccine using three different administration methods and the immune responses were measured.

Limitations

The study was conducted in a mouse model, which may not fully replicate human responses.

Participant Demographics

Inbred female C57BL/6 mice, aged 6–8 weeks.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1479-0556-7-2

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