Combining Cyclosporin A and Itraconazole to Inhibit Endothelial Cell Growth
Author Information
Author(s): Nacev Benjamin A., Liu Jun O.
Primary Institution: Johns Hopkins University School of Medicine
Hypothesis
Can existing clinical drugs be combined to synergistically inhibit endothelial cell proliferation and tube formation?
Conclusion
The combination of cyclosporin A and itraconazole significantly inhibits endothelial cell proliferation and tube formation more effectively than either drug alone.
Supporting Evidence
- The combination of cyclosporin A and itraconazole reduced the IC50 dose of each drug by 3 to 9 fold.
- CsA and Ita were shown to synergistically inhibit tube network size and sprout formation.
- The combination selectively targeted endothelial cells without significantly affecting other cell types.
Takeaway
Researchers found that using two existing drugs together can stop certain cells from growing better than using just one drug.
Methodology
The study screened 741 drug combinations for their ability to inhibit endothelial cell proliferation and tube formation using high-throughput assays.
Limitations
The study primarily focused on in vitro models, which may not fully represent in vivo conditions.
Statistical Information
P-Value
0.043
Confidence Interval
95% CI, 2.6 nM, 8.0 nM
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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