PTEN and Rapamycin Inhibit K562 Cell Growth
Author Information
Author(s): Cheng Zhi Y, Guo Xiao L, Yang Xiao Y, Niu Zhi Y, Li Shi H, Wang Su Y, Chen Hao, Pan Ling
Primary Institution: The Second Hospital of Hebei Medical University
Hypothesis
The study investigates the regulatory effects of the tumor-suppressing gene PTEN on the mTOR signaling pathway and its impact on the growth inhibition and apoptosis of K562 cells.
Conclusion
PTEN and rapamycin inhibit mTOR expression, suggesting a potential synergistic effect on leukemia treatment.
Supporting Evidence
- PTEN gene transfection significantly inhibited K562 cell growth.
- Rapamycin treatment combined with PTEN transfection led to a higher apoptosis rate in K562 cells.
- The expression levels of PTEN mRNA increased after transfection.
- mTOR mRNA levels decreased in K562 cells after PTEN transfection.
Takeaway
Scientists found that a gene called PTEN and a drug called rapamycin can work together to stop cancer cells from growing.
Methodology
K562 cells were transfected with an adenovirus-PTEN vector and treated with rapamycin, followed by measuring cell proliferation and apoptosis.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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