Comparative Study of Stem Cells in Fat and Bone Development
Author Information
Author(s): Marcel Scheideler, Christian Elabd, Laure-Emmanuelle Zaragosi, Chiara Chiellini, Hubert Hackl, Fatima Sanchez-Cabo, Sunaina Yadav, Kalina Duszka, Gerald Friedl, Christine Papak, Andreas Prokesch, Reinhard Windhager, Gerard Ailhaud, Christian Dani, Ez-Zoubir Amri, Zlatko Trajanoski
Primary Institution: Institute for Genomics and Bioinformatics, Graz University of Technology
Hypothesis
How do human multipotent adipose tissue-derived stem cells regulate the balance between fat and bone cell development?
Conclusion
The study identifies gene repression as a key mechanism before cell commitment and highlights 65 candidate genes involved in the adipocyte/osteoblast balance.
Supporting Evidence
- 1606 genes were found to be differentially expressed between adipogenesis and osteoblastogenesis.
- Gene repression was most prevalent prior to commitment in both lineages.
- Computational analyses suggested that gene repression is mediated by miRNAs.
- Four candidate genes were studied in detail: LXRα, PLTP, COUP-TF1, and TMEM135.
Takeaway
This study looks at how certain stem cells can turn into fat or bone cells and finds that some genes are turned off before they make that choice.
Methodology
The study used large-scale gene expression profiling and computational analyses to examine gene expression changes in stem cells differentiating into fat and bone cells.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website