Identifying Genes That Affect Transgene Variegation in Mice
Author Information
Author(s): Alyson Ashe, Daniel K Morgan, Nadia C Whitelaw, Timothy J Bruxner, Nicola K Vickaryous, Liza L Cox, Natalie C Butterfield, Carol Wicking, Marnie E Blewitt, Sarah J Wilkins, Gregory J Anderson, Timothy C Cox, Emma Whitelaw
Primary Institution: Queensland Institute of Medical Research
Hypothesis
Can a genome-wide screen identify modifiers of transgene variegation in mice?
Conclusion
The study identified four new modifiers of transgene variegation, highlighting the importance of epigenetic reprogramming in embryonic development.
Supporting Evidence
- The screen identified four new modifiers, MommeD7-D10.
- Homozygosity for mutations was linked to severe developmental abnormalities.
- Reduction in Baz1b levels resulted in craniofacial features similar to Williams syndrome.
- The study validated the effectiveness of the screening design.
Takeaway
Scientists looked for genes that change how a gene shows up in mice and found some that are really important for how embryos develop.
Methodology
The study used an N-ethyl-N-nitrosourea screen to identify mutations affecting transgene expression in mice.
Potential Biases
Potential bias in the selection of mutations and their phenotypic characterization.
Limitations
The study primarily focused on a specific set of mutations and may not encompass all potential modifiers.
Participant Demographics
Mice used in the study were primarily from the FVB/NJ inbred strain.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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