Identifying Thyroid Hormone Receptor Binding Sites in Mouse Brain Development
Author Information
Author(s): Dong Hongyan, Yauk Carole L., Rowan-Carroll Andrea, You Seo-Hee, Zoeller R. Thomas, Lambert Iain, Wade Michael G.
Primary Institution: Health Canada, Ottawa, Ontario, Canada
Hypothesis
Thyroid hormone (TH) is critical to normal brain development, but the mechanisms operating in this process are poorly understood.
Conclusion
The study identified 91 genes with TR binding sites in the developing mouse cerebellum, providing insight into the role of thyroid hormone in neurodevelopment.
Supporting Evidence
- The study identified 91 genes with TR binding sites.
- Approximately half of the binding sites were located in introns.
- The expression of 4 novel TH target genes correlated with TH levels in hypothyroid and hyperthyroid animals.
- ChIP-on-chip results were confirmed for 10 of the 13 binding fragments using ChIP-PCR.
Takeaway
This study looked at how thyroid hormones help the brain grow in baby mice and found many genes that are affected by these hormones.
Methodology
Chromatin immunoprecipitation combined with DNA microarray analysis (ChIP-on-chip) was used to identify direct gene targets of TH in the cerebellum of postnatal day 15 mice.
Limitations
The study has a false discovery rate of approximately 23% for the identified binding sites.
Participant Demographics
C57BL/6 mice, specifically male pups at postnatal day 15.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website