Structure-Guided Comparative Analysis of Proteins: Principles, Tools, and Applications for Predicting Function
2008

Ten-Step Procedure for Predicting Protein Function

publication Evidence: moderate

Author Information

Author(s): Mazumder, Vasudevan, Sona

Primary Institution: Georgetown University Medical Center

Hypothesis

Can a systematic ten-step procedure improve the prediction of protein functions from uncharacterized sequences?

Conclusion

The study outlines a ten-step procedure that can be used to infer the biological function of uncharacterized proteins based on comparative analysis.

Supporting Evidence

  • The procedure is guided by a percent-identity scale to assess functional conservation.
  • Functional predictions are based on comparative analysis of sequences and structures.
  • Experimental validation is crucial for reliable functional inference.

Takeaway

This study provides a simple ten-step guide to help scientists figure out what a protein does, even if they don't know much about it yet.

Methodology

The article describes a ten-step procedure for functional inference of proteins, utilizing sequence and structural comparisons.

Potential Biases

Potential bias in selecting databases and tools based on the authors' familiarity.

Limitations

The procedure may not cover all methods for functional inference and relies on the availability of related experimental data.

Digital Object Identifier (DOI)

10.1371/journal.pcbi.1000151

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