Roles of the Mono-ADP-Ribosyltransferase Arr in Mycobacterial DNA Damage Response
Author Information
Author(s): Christina L. Stallings, Linda Chu, Lucy X. Li, Michael S. Glickman
Primary Institution: Washington University School of Medicine
Hypothesis
What role does the mono-ADP-ribosyltransferase Arr play in the mycobacterial DNA damage response?
Conclusion
The study identifies both catalytic and non-catalytic roles for Arr in regulating gene expression during DNA damage in mycobacteria.
Supporting Evidence
- Arr is transcriptionally induced following DNA damage and cellular stress.
- Arr is necessary for suppression of ribosomal protein genes during DNA damage.
- Arr has both catalytic and non-catalytic roles in the DNA damage response.
Takeaway
Mycobacteria have a special protein called Arr that helps them deal with DNA damage. It can work in two ways: one that needs its special activity and one that doesn't.
Methodology
The study used whole genome transcriptional profiling and microarray analyses to assess gene expression changes in Mycobacterium smegmatis during DNA damage.
Limitations
The study did not identify endogenous targets of ADP-ribosylation in mycobacteria.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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