Diabetic Impairment of C-Kit+ Bone Marrow Stem Cells Involves the Disorders of Inflammatory Factors, Cell Adhesion and Extracellular Matrix Molecules
2011

Diabetes Impairs Bone Marrow Stem Cells

publication Evidence: moderate

Author Information

Author(s): Li Tao-Sheng, Ikeda Satoshi, Kubo Masayuki, Ohshima Mako, Kurazumi Hiroshi, Takemoto Yoshihiro, Ueda Kazuhiro, Hamano Kimikazu

Primary Institution: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Science, Nagasaki, Japan

Hypothesis

The study investigates the mechanisms responsible for diabetes-related functional impairment of bone marrow stem cells.

Conclusion

Diabetes leads to an increase in inflammatory factors and changes in extracellular matrix and adhesion molecules in bone marrow stem cells, contributing to their functional impairment.

Supporting Evidence

  • Diabetic mice had significantly fewer c-kit+ stem cells compared to healthy controls.
  • c-kit+ stem cells from diabetic mice showed lower potency of endothelial differentiation.
  • Diabetes increased the expression of inflammatory-related factors in c-kit+ bone marrow stem cells.

Takeaway

Diabetes makes special cells in the bone marrow not work as well, which can cause problems in healing and repairing the body.

Methodology

Bone marrow cells were collected from diabetic and healthy mice, and c-kit+ stem cells were purified for analysis of inflammatory factors and cell adhesion molecules.

Limitations

The study did not identify the critical factors inducing functional impairment and only screened three pathways.

Participant Demographics

12-week-old male C57BLKS/J mice, including diabetic and healthy control groups.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025543

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