Derivation of Rhesus Monkey Parthenogenetic Embryonic Stem Cells and Its MicroRNA Signature
Author Information
Author(s): Wei Qiang, Sun Zhenghua, He Xiechao, Tan Tao, Lu Bin, Guo Xiangyu, Su Bing, Ji Weizhi
Primary Institution: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
Hypothesis
The study aims to derive and characterize the microRNA signature of parthenogenetic embryonic stem cells from rhesus monkeys.
Conclusion
The derived parthenogenetic embryonic stem cells exhibit unique microRNA signatures and share many properties with other primate embryonic stem cells.
Supporting Evidence
- Two novel parthenogenetic embryonic stem cell lines were successfully derived from rhesus monkey blastocysts.
- The derived stem cells maintained a euploid karyotype after long-term culture.
- These stem cells could differentiate into all three germ layers in vitro.
- 91 novel microRNAs were identified in the parthenogenetic embryonic stem cells.
Takeaway
Scientists created special stem cells from monkey embryos that can help us understand how cells grow and develop, and they found some unique tiny molecules that help control this process.
Methodology
The researchers derived two novel parthenogenetic embryonic stem cell lines from rhesus monkey blastocysts and characterized their microRNA profiles using Solexa deep sequencing.
Potential Biases
There may be biases related to the limited genetic diversity of the rhesus monkeys used in the study.
Limitations
The study is limited by the small number of parthenogenetic embryonic stem cell lines derived and the potential variability in microRNA expression.
Participant Demographics
Rhesus monkeys (Macaca mulatta) were used as the model organism.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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