Broad Antiviral Activity of Carbohydrate-Binding Agents against the Four Serotypes of Dengue Virus in Monocyte-Derived Dendritic Cells
2011

Broad Antiviral Activity of Carbohydrate-Binding Agents against Dengue Virus

publication Evidence: high

Author Information

Author(s): Alen Marijke M. F. De Burghgraeve, Tine Kaptein, Suzanne J. F. Balzarini, Jan Neyts, Johan Schols, Dominique Schols

Primary Institution: Katholieke Universiteit Leuven

Hypothesis

Can carbohydrate-binding agents (CBAs) effectively inhibit the replication of all four serotypes of dengue virus in dendritic cells?

Conclusion

Carbohydrate-binding agents demonstrated broad-spectrum antiviral activity against all four dengue virus serotypes in monocyte-derived dendritic cells.

Supporting Evidence

  • The CBAs were significantly more effective in monocyte-derived dendritic cells compared to Raji/DC-SIGN+ cells.
  • CBAs prevented the cellular activation and differentiation process induced by dengue virus infection.
  • CBAs acted at an early step of dengue virus infection by binding to the viral envelope.

Takeaway

This study found that certain plant extracts can help stop dengue virus from making people sick by blocking the virus from entering important immune cells.

Methodology

The study evaluated the antiviral activity of various carbohydrate-binding agents against dengue virus in monocyte-derived dendritic cells using flow cytometry.

Limitations

The study primarily focused on in vitro models, which may not fully replicate in vivo conditions.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021658

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