Reducing Deoxynivalenol in Barley Ethanol Co-Products
Author Information
Author(s): Khatibi Piyum A, Montanti Justin, Nghiem Nhuan P, Hicks Kevin B, Berger Greg, Brooks Wynse S, Griffey Carl A, Schmale David G III
Primary Institution: Virginia Tech, Department of Plant Pathology, Physiology and Weed Science
Hypothesis
Two enzymes (FgTRI101 and FfTRI201) would reduce DON in DDGS resulting from a series of small-scale barley ethanol fermentations.
Conclusion
Using yeast expressing trichothecene 3-O-acetyltransferases can effectively convert DON to 3ADON, reducing its concentration in DDGS.
Supporting Evidence
- DON levels were concentrated 1.6 to 8.2 times in DDGS compared with the starting ground grain.
- FgTRI101 converted 9.2% to 55.3% of the DON to 3ADON.
- FfTRI201 was more effective at acetylating DON than FgTRI101.
Takeaway
This study shows that special yeast can change a harmful substance in barley into a less harmful one during the process of making ethanol.
Methodology
Yeast strains were transformed with genes for trichothecene 3-O-acetyltransferases and used in small-scale ethanol fermentations of barley.
Potential Biases
Potential bias in the selection of barley cultivars and yeast strains used in the study.
Limitations
The study did not explore the long-term effects of consuming DDGS with reduced DON levels.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website