Role of 1,3-Propanediol Dehydrogenases in Lactobacillus reuteri
Author Information
Author(s): Marc J A Stevens, Sabine Vollenweider, Leo Meile, Christophe Lacroix
Primary Institution: ETH Zurich
Hypothesis
What is the impact of 1,3-propanediol dehydrogenases on central metabolism and 3-hydroxypropionaldehyde production in Lactobacillus reuteri?
Conclusion
The enzyme encoded by lr_0030 is crucial for 3-HPA conversion in growing L. reuteri cells, while lr_1734 is important for 3-HPA production in non-growing cells.
Supporting Evidence
- The enzyme encoded by lr_0030 is essential for 3-HPA conversion during exponential growth.
- Deletion of lr_1734 resulted in a higher ratio of 3-HPA to 1,3-PDO during production.
- Both PDHs were identified as important for different growth conditions in L. reuteri.
Takeaway
Lactobacillus reuteri has two enzymes that help it turn glycerol into useful products, and they work differently depending on whether the bacteria are growing or not.
Methodology
Gene deletion mutants were constructed to study the role of two putative PDHs in L. reuteri, and growth behavior and metabolite production were analyzed.
Limitations
The study primarily focuses on two specific genes and their roles, which may not encompass all factors affecting 3-HPA and 1,3-PDO production.
Statistical Information
P-Value
0.02
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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