Crystallization and preliminary X-ray diffraction analyses of several forms of the CfaB major subunit of enterotoxigenic Escherichia coli CFA/I fimbriae
2009
Structure of CfaB Subunit of E. coli Fimbriae
publication
Evidence: high
Author Information
Author(s): Li Yong-Fu, Poole Steven, Rasulova Fatima, McVeigh Annette L., Savarino Stephen J., Xia Di
Primary Institution: National Cancer Institute, NIH
Hypothesis
To elucidate the structure of CFA/I fimbriae at atomic resolution.
Conclusion
The study successfully determined the crystal structures of three fusion proteins related to the CfaB subunit of E. coli CFA/I fimbriae.
Supporting Evidence
- Three recombinant proteins were generated to study the structure of CFA/I fimbriae.
- CfaEB, CfaBB, and CfaBBB were successfully crystallized and analyzed.
- The structures were determined using the molecular-replacement method.
Takeaway
Scientists created special proteins to understand how a part of E. coli sticks to our intestines, which helps them learn about how this bacteria causes diarrhea.
Methodology
Three fusion proteins were generated and crystallized to determine their structures using X-ray diffraction.
Limitations
The study does not mention specific limitations.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website