Identifying Proteins Related to Pancreatic Cancer Invasion
Author Information
Author(s): Naomi Walsh, Norma O'Donovan, Susan Kennedy, Michael Henry, Paula Meleady, Martin Clynes, Paul Dowling
Primary Institution: National Institute for Cellular Biotechnology, Dublin City University
Hypothesis
What proteins are involved in the invasion of pancreatic cancer cells?
Conclusion
The study identified proteins that may play a role in the invasive behavior of pancreatic cancer, which could help in developing new treatments.
Supporting Evidence
- Sixty proteins were identified as differentially expressed between high and low invasive pancreatic cancer cell lines.
- STIP1 and VIM knockdown reduced invasion and proliferation in the highly invasive pancreatic cancer cell line.
- Increased STIP1 expression was observed in pancreatic tumor tissue compared to normal pancreas.
Takeaway
Researchers looked at two types of pancreatic cancer cells to find out which proteins help them invade more. They found some proteins that could be important for new cancer treatments.
Methodology
Proteomic profiling using 2D-DIGE followed by MALDI-TOF MS to analyze protein expression in two clonal populations of pancreatic cancer cells.
Limitations
The study is based on in vitro models and may not fully represent in vivo conditions.
Participant Demographics
[{"sex":"M","age":48,"tumour_type":"Adenocarcinoma, ductal","tumour_grade":"Moderately differentiated","lymph_node_status":"+ve"},{"sex":"F","age":43,"tumour_type":"Adenocarcinoma","tumour_grade":"Well differentiated","lymph_node_status":"-ve"},{"sex":"F","age":67,"tumour_type":"Adenocarcinoma","tumour_grade":"Well differentiated","lymph_node_status":"-ve"},{"sex":"M","age":36,"tumour_type":"Adenocarcinoma","tumour_grade":"Poorly differentiated","lymph_node_status":"+ve"},{"sex":"M","age":67,"tumour_type":"Squamous cell","tumour_grade":"Poorly differentiated","lymph_node_status":"No nodes"}]
Statistical Information
P-Value
p = 0.0002
Statistical Significance
p ≤ 0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website