Restriction Endonucleases that Bridge and Excise Two Recognition Sites from DNA
2007
Restriction Endonucleases that Bridge and Excise Two Recognition Sites from DNA
Sample size: 10
publication
Evidence: high
Author Information
Author(s): Marshall Jacqueline J.T., Gowers Darren M., Halford Stephen E.
Primary Institution: University of Bristol
Hypothesis
Whether Type IIB enzymes require interaction with two recognition sites to cleave DNA.
Conclusion
Most Type IIB restriction enzymes cleave DNA more rapidly when interacting with two recognition sites compared to one.
Supporting Evidence
- Most Type IIB enzymes cleave two-site substrates more rapidly than one-site substrates.
- BcgI interacts with two copies of its recognition sequence before cutting DNA.
- Type IIB enzymes can act in trans by forming a complex bridging one recognition site on a plasmid and another on an oligoduplex.
Takeaway
Some enzymes need to grab onto two spots on the DNA before they can cut it, which helps them work better.
Methodology
Ten Type IIB nucleases were tested against DNA substrates with one or two copies of the requisite sequences.
Limitations
The study primarily focused on Type IIB enzymes and may not represent all restriction enzymes.
Digital Object Identifier (DOI)
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