Regulation of p73 by Hck through kinase-dependent and independent mechanisms
2007
How Hck Regulates p73 Activity
publication
Evidence: moderate
Author Information
Author(s): Paliwal Preeti, Radha Vegesna, Swarup Ghanshyam
Primary Institution: Centre for Cellular and Molecular Biology, Hyderabad, India
Hypothesis
Hck regulates p73 through kinase-dependent and independent mechanisms.
Conclusion
Hck interacts with p73 and regulates its activity, inhibiting p73-dependent transcriptional activation and apoptosis.
Supporting Evidence
- Hck interacts with p73 in vivo and in vitro.
- Hck co-expression leads to tyrosine phosphorylation of p73.
- Hck stabilizes p73 protein levels in a kinase-dependent manner.
- Hck inhibits p73-mediated transcriptional activation of target genes.
- Hck's effect on p73 is independent of its kinase activity.
- Hck interacts with YAP, which is required for p73-induced activation of the Ipaf promoter.
Takeaway
Hck is a protein that can change how another protein called p73 works, making it less effective at doing its job, which includes helping cells die when they should.
Methodology
The study used co-immunoprecipitation, western blotting, and various assays to analyze the interaction and effects of Hck on p73.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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