Detection of Glycoprotein of Burkholderia pseudomallei
1998
Detection of Glycoprotein of Burkholderia pseudomallei
Sample size: 61
publication
Evidence: moderate
Author Information
Author(s): Natalja P. Khrapova, Nikolay G. Tikhonov, Yelena V. Prokhvatilova
Primary Institution: Volgograd Antiplague Research Institute
Hypothesis
The study aims to develop a reliable detection method for the glycoprotein Ag8 of Burkholderia pseudomallei.
Conclusion
The developed immunoenzyme test system can effectively detect the glycoprotein Ag8 in various strains of Burkholderia pseudomallei.
Supporting Evidence
- The glycoprotein Ag8 is present in different strains of Burkholderia pseudomallei and B. mallei but not in other Burkholderia species.
- The sensitivity of the immunoenzyme test system was determined with a standard antigen sample.
- Maximal sensitivity was noted when monoclonal antibodies were used as catching antibodies.
Takeaway
Scientists created a test to find a specific protein from a germ that causes a serious disease, helping doctors diagnose it better.
Methodology
The study used a sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies to detect Ag8.
Limitations
The study does not specify the limitations of the test system.
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