Reassessing the Role of APOBEC3G in HIV-1 Infection of Quiescent CD4+ T-Cells
Author Information
Author(s): Kamata Masakazu, Nagaoka Yoshiko, Chen Irvin S. Y.
Primary Institution: David Geffen School of Medicine, University of California Los Angeles
Hypothesis
Does APOBEC3G play a role in restricting HIV-1 infection in quiescent CD4+ T-cells?
Conclusion
The study concludes that APOBEC3G does not play a significant role in restricting HIV-1 infection in quiescent CD4+ T-cells.
Supporting Evidence
- APOBEC3G knock-down did not enhance HIV-1 infection in quiescent CD4+ T-cells.
- Three different siRNAs targeting APOBEC3G were tested with no significant effect on HIV-1 infection.
- Results were consistent across six different donors.
Takeaway
Scientists wanted to see if a protein called APOBEC3G stops HIV from infecting certain immune cells, but they found it doesn't help at all.
Methodology
The study used siRNA to knock down APOBEC3G in quiescent CD4+ T-cells and measured HIV-1 infection levels.
Potential Biases
Potential biases may arise from differences in cell source and culture conditions.
Limitations
The study's results were not consistent with previous findings, and the reasons for discrepancies were not fully understood.
Participant Demographics
Quiescent CD4+ T-cells derived from peripheral blood mononuclear cells of healthy human donors.
Statistical Information
P-Value
0.00005
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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