pH Landscapes in an Early Dental Biofilm Model
Author Information
Author(s): Schlafer Sebastian, Raarup Merete K., Meyer Rikke L., Sutherland Duncan S., Dige Irene, Nyengaard Jens R., Nyvad Bente
Primary Institution: Aarhus University, Aarhus, Denmark
Hypothesis
The study aims to develop a laboratory biofilm model that mimics the bacterial community present during early acidogenic stages of the caries process.
Conclusion
The designed biofilm model provides valuable insights into the pH landscape of living biofilms and their metabolic processes.
Supporting Evidence
- The biofilm model mimics the bacterial community present during early acidogenic stages of caries.
- Different pH microenvironments were observed in the biofilms, which persisted over several hours.
- Real-time pH analysis provided insights into the metabolic processes of the biofilm.
Takeaway
Researchers created a model to study how bacteria in dental plaque change the pH, which helps us understand tooth decay better.
Methodology
The study used a five-species biofilm model with real-time pH monitoring using a ratiometric fluorescent probe.
Limitations
The model may not fully replicate the complexity of the oral environment.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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