Immunogenic Eimeria tenella Glycosylphosphatidylinositol-Anchored Surface Antigens (SAGs) Induce Inflammatory Responses in Avian Macrophages
2011

Eimeria tenella Surface Antigens and Chicken Immune Response

publication Evidence: moderate

Author Information

Author(s): Chow Yock-Ping, Wan Kiew-Lian, Blake Damer P., Tomley Fiona, Nathan Sheila

Primary Institution: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia

Hypothesis

The study investigates the role of glycosylphosphatidylinositol-anchored surface antigens (SAGs) of Eimeria tenella in stimulating immune responses in chickens.

Conclusion

Some E. tenella SAGs can modulate chicken innate and humoral immune responses, with certain SAGs linked to increased pathogenicity.

Supporting Evidence

  • E. tenella SAGs are expressed during the development of second-generation merozoites.
  • Treatment with specific SAGs induced high levels of macrophage nitric oxide production.
  • SAGs 4, 5, and 12 suppressed IL-12 and IFN-γ expression while elevating IL-10 levels.

Takeaway

The proteins from a parasite called Eimeria tenella can change how chickens' immune systems react, which might help the parasite survive better.

Methodology

The study involved expressing ten SAGs as recombinant proteins in E. coli, treating chicken macrophages with these proteins, and measuring changes in nitric oxide production and cytokine mRNA expression.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025233

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