Comparison between the HCV IRES domain IV RNA structure and the Iron Responsive Element
2009
HCV IRES Domain IV RNA Structure and Iron Responsive Element Comparison
publication
Evidence: moderate
Author Information
Author(s): Tumban Ebenezer, Painter Jenna M, Lott William B
Primary Institution: New Mexico State University
Hypothesis
Does the HCV IRES domain IV RNA structure function as an authentic iron responsive element?
Conclusion
The HCV IRES dIV RNA structure is not an authentic IRE.
Supporting Evidence
- The HCV IRES dIV structure does not interact with the iron regulatory protein 1 (IRP1) in vitro.
- Mutational analysis suggested that IRP1 cannot accommodate the shape of the wild type HCV IRES dIV RNA structure.
- The study indicates that the HCV IRES dIV RNA structure is not an authentic IRE.
Takeaway
The study looked at a part of the hepatitis C virus and found that it doesn't work like a specific structure that helps control iron levels in cells.
Methodology
Electrophoretic mobility shift assays and systematic RNA mutagenesis were used to evaluate binding interactions.
Limitations
The study does not identify a cellular or viral factor that could fulfill the IRP function for HCV.
Digital Object Identifier (DOI)
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