Complete Nucleotide Analysis of the Infectious Bronchitis Virus Strain Md27
Author Information
Author(s): Ammayappan Arun, Vakharia Vikram N.
Primary Institution: University of Maryland
Hypothesis
The study aims to understand the genetic diversity and phylogenetic relationship of the Md27 strain of the infectious bronchitis virus with other strains.
Conclusion
The Md27 strain is a chimera containing different gene segments that are most closely related to the SE17, Conn, and JMK strains.
Supporting Evidence
- The spike gene of Md27 exhibits 97% identity with the SE17 strain.
- Phylogenetic analysis shows Md27 is closely related to several other IBV strains.
- The study provides evidence for genomic mutations and intergenic recombination in the evolution of IBV strain Md27.
Takeaway
Scientists looked at the genes of a virus that makes chickens sick and found that it has parts from different viruses, which helps explain how it changes over time.
Methodology
The complete nucleotide sequence of the 3′ end coding region of Md27 was determined and compared with other IBV strains and coronaviruses.
Limitations
The study is limited by the availability of complete sequences for other strains for comparison.
Digital Object Identifier (DOI)
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