Study of Bordetella bronchiseptica Gene Expression Using Microarrays
Author Information
Author(s): Tracy L. Nicholson
Primary Institution: National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, USA
Hypothesis
How does the BvgAS system regulate gene expression in Bordetella bronchiseptica?
Conclusion
The study provides a comprehensive view of the BvgAS regulon in Bordetella bronchiseptica, identifying 1,668 Bvg-regulated genes.
Supporting Evidence
- The study identified 1,668 Bvg-regulated genes, significantly expanding the known BvgAS regulon.
- Quantitative real-time PCR provided independent verification of the microarray results.
- The long-oligonucleotide microarray was validated by comparing gene expression profiles of two Bordetella strains.
Takeaway
Researchers created a special tool to study how a bacteria called Bordetella bronchiseptica changes its genes when it gets sick, finding many new genes that help it survive.
Methodology
A long-oligonucleotide microarray was designed and validated by comparing gene expression profiles between two strains of Bordetella bronchiseptica.
Limitations
Some Bvg-activated genes may have been missed due to the strain differences used in the analysis.
Statistical Information
P-Value
<0.1
Statistical Significance
p<0.1
Digital Object Identifier (DOI)
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